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Immortalization of human hepatocytes by temperature-sensitive SV40 large-T antigen

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References

  • Fox, I. J.; Chowdhury, N. R.; Gupta, S.; Kondapalli, R.; Schilsky, M. L.; Stockert, R. J.; Chowdhury, J. R. Conditional immortalisation of Gunn rat hepatocytes: an ex vivo model for evaluating methods for bilirubin-UDP-glucuronosyltransferase gene transfer. Hepatology 21:837–846; 1995.

    PubMed  CAS  Google Scholar 

  • Georgoff, I.; Secott, T.; Isom, H. C. Effect of simian virus 40 infection on albumin production by hepatocytes cultured in chemically defined medium and plated on collagen and non-collagen attachment surfaces. J. Biol. Chem. 259:9595–9602; 1984.

    PubMed  CAS  Google Scholar 

  • Livingstone, C.; MacDonald, C.; Willet, B.; Houslay, M. D. Analysis of the adenylate cyclase signalling system, and alterations induced by culture with insulin, in a novel SV40-DNA-immortalized hepatocyte cell line (P9 cells). Biochem. J. 300:835–842; 1994.

    PubMed  CAS  Google Scholar 

  • Moll, R.; Franke, W. W.; Schiller, D. L.; Geiger, B.; Krepler, R. The catalogue of human cytokeratins: patterns of expression in normal epithelia, tumours and cultured cells. Cell 31:11–24; 1982.

    Article  PubMed  CAS  Google Scholar 

  • Nakamura, K.; Endo, F.; Ueno, T.; Awata, H.; Tanoue, A.; Matsuda, I. Excess copper and ceruloplasmin biosynthesis in long-term cultured hepatocytes from Long-Evans Cinnamon (LEC) rats, a model of Wilson disease. J. Biol. Chem. 270:7656–7660; 1995.

    Article  PubMed  CAS  Google Scholar 

  • Pfeifer, A. M. A.; Cole, K. E.; Smoot, D. T., et al. Simian virus 40 large tumour antigen-immortalised normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90:5123–5127; 1993.

    Article  PubMed  CAS  Google Scholar 

  • Spath, G. F.; Weiss, M. C. Hepatocyte nuclear factor 4 provokes expression of epithelial marker genes, acting as a morphogen in dedifferentiated hepatoma cells. J. Cell Biol. 140:935–946; 1998.

    Article  PubMed  CAS  Google Scholar 

  • Stamps, A. C.; Davies, S. C.; Burman, J.; O'Hare, M. J. Analysis of proviral integration in human mammary epithelial cell lines immortalised by retroviral infection with a temperature-sensitive SV40 T-antigen construct. Int. J. Cancer 57:865–874; 1994.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Royal Postgraduate Medical School, London, UK

    M. Smalley, K. Leiper, R. Tootle, P. McCloskey & H. Hodgson

  2. Department of Surgery, University College, London, UK

    M. J. O'Hare

Authors
  1. M. Smalley
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  2. K. Leiper
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  3. R. Tootle
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  4. P. McCloskey
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  5. M. J. O'Hare
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  6. H. Hodgson
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Corresponding author

Correspondence to H. Hodgson.

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Smalley, M., Leiper, K., Tootle, R. et al. Immortalization of human hepatocytes by temperature-sensitive SV40 large-T antigen. In Vitro Cell.Dev.Biol.-Animal 37, 166–168 (2001). https://doi.org/10.1290/1071-2690(2001)037<0166:IOHHBT>2.0.CO;2

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  • DOI: https://doi.org/10.1290/1071-2690(2001)037<0166:IOHHBT>2.0.CO;2

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